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pro-long gold anti-fade reagent with dapi  (Thermo Fisher)


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    Thermo Fisher pro-long gold anti-fade reagent with dapi
    Pro Long Gold Anti Fade Reagent With Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro+long+anti-fade+reagent+dapi/prolong+diamond+antifade+mountant/pm35833925-274-0-6
    Average 90 stars, based on 1 article reviews
    pro-long gold anti-fade reagent with dapi - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells.
    Article Snippet: After washing, samples were stained with AlexaFluor-488 or AlexaFluor-546 secondary antibodies in the dark, counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milano, Italy).

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: Finally, after washes, the samples were mounted and nuclei counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milan, Italy).

    Article Title: The role of 3D microenvironmental organization in MCF-7 epithelial-mesenchymal transition after 7 culture days.
    Article Snippet: We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations.. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points.. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7.

    Article Title: Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds
    Article Snippet: a Department of Chemistry ‘‘G.. Ciamician’’ and National Consortium of Materials Science and Technology (INSTM, RU Bologna), University of Bologna, Bologna, Italy b Laboratory of Cellular and Molecular Engineering ‘‘S.. Cavalcanti’’, University of Bologna, Cesena, Italy c Health Sciences and Technologies – Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano dell’Emilia, Italy d National Institute for Cardiovascular Research (INRC), University of Bologna, Bologna, Italy e Department of Anaestesiological and Surgical Sciences, University of Bologna, Bologna, Italy f Consiglio Nazionale delle Ricerche – Istituto per lo Studio dei Materiali Nanostrutturati (CNR–ISMN), Bologna, Italy g Laboratorio di NanoBiotecnologie (NaBi) – Istituto Ortopedico Rizzoli, Bologna, Italy h Clinical Department of Radiological and Histocytopathological Sciences, University of Bologna, Bologna, Italy

    Real-time Polymerase Chain Reaction:

    Article Title: Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells.
    Article Snippet: After washing, samples were stained with AlexaFluor-488 or AlexaFluor-546 secondary antibodies in the dark, counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milano, Italy).

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: Finally, after washes, the samples were mounted and nuclei counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milan, Italy).

    Article Title: The role of 3D microenvironmental organization in MCF-7 epithelial-mesenchymal transition after 7 culture days.
    Article Snippet: We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations.. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points.. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7.

    Article Title: Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds
    Article Snippet: a Department of Chemistry ‘‘G.. Ciamician’’ and National Consortium of Materials Science and Technology (INSTM, RU Bologna), University of Bologna, Bologna, Italy b Laboratory of Cellular and Molecular Engineering ‘‘S.. Cavalcanti’’, University of Bologna, Cesena, Italy c Health Sciences and Technologies – Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano dell’Emilia, Italy d National Institute for Cardiovascular Research (INRC), University of Bologna, Bologna, Italy e Department of Anaestesiological and Surgical Sciences, University of Bologna, Bologna, Italy f Consiglio Nazionale delle Ricerche – Istituto per lo Studio dei Materiali Nanostrutturati (CNR–ISMN), Bologna, Italy g Laboratorio di NanoBiotecnologie (NaBi) – Istituto Ortopedico Rizzoli, Bologna, Italy h Clinical Department of Radiological and Histocytopathological Sciences, University of Bologna, Bologna, Italy

    Immunofluorescence:

    Article Title: Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells.
    Article Snippet: After washing, samples were stained with AlexaFluor-488 or AlexaFluor-546 secondary antibodies in the dark, counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milano, Italy).

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: Finally, after washes, the samples were mounted and nuclei counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milan, Italy).

    Article Title: The role of 3D microenvironmental organization in MCF-7 epithelial-mesenchymal transition after 7 culture days.
    Article Snippet: We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations.. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points.. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7.

    Article Title: Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds
    Article Snippet: a Department of Chemistry ‘‘G.. Ciamician’’ and National Consortium of Materials Science and Technology (INSTM, RU Bologna), University of Bologna, Bologna, Italy b Laboratory of Cellular and Molecular Engineering ‘‘S.. Cavalcanti’’, University of Bologna, Cesena, Italy c Health Sciences and Technologies – Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano dell’Emilia, Italy d National Institute for Cardiovascular Research (INRC), University of Bologna, Bologna, Italy e Department of Anaestesiological and Surgical Sciences, University of Bologna, Bologna, Italy f Consiglio Nazionale delle Ricerche – Istituto per lo Studio dei Materiali Nanostrutturati (CNR–ISMN), Bologna, Italy g Laboratorio di NanoBiotecnologie (NaBi) – Istituto Ortopedico Rizzoli, Bologna, Italy h Clinical Department of Radiological and Histocytopathological Sciences, University of Bologna, Bologna, Italy

    Standard Deviation:

    Article Title: Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells.
    Article Snippet: After washing, samples were stained with AlexaFluor-488 or AlexaFluor-546 secondary antibodies in the dark, counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milano, Italy).

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: Finally, after washes, the samples were mounted and nuclei counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milan, Italy).

    Article Title: The role of 3D microenvironmental organization in MCF-7 epithelial-mesenchymal transition after 7 culture days.
    Article Snippet: We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations.. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points.. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7.

    Article Title: Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds
    Article Snippet: a Department of Chemistry ‘‘G.. Ciamician’’ and National Consortium of Materials Science and Technology (INSTM, RU Bologna), University of Bologna, Bologna, Italy b Laboratory of Cellular and Molecular Engineering ‘‘S.. Cavalcanti’’, University of Bologna, Cesena, Italy c Health Sciences and Technologies – Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano dell’Emilia, Italy d National Institute for Cardiovascular Research (INRC), University of Bologna, Bologna, Italy e Department of Anaestesiological and Surgical Sciences, University of Bologna, Bologna, Italy f Consiglio Nazionale delle Ricerche – Istituto per lo Studio dei Materiali Nanostrutturati (CNR–ISMN), Bologna, Italy g Laboratorio di NanoBiotecnologie (NaBi) – Istituto Ortopedico Rizzoli, Bologna, Italy h Clinical Department of Radiological and Histocytopathological Sciences, University of Bologna, Bologna, Italy

    Fluorescence:

    Article Title: Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells.
    Article Snippet: After washing, samples were stained with AlexaFluor-488 or AlexaFluor-546 secondary antibodies in the dark, counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milano, Italy).

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: Finally, after washes, the samples were mounted and nuclei counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milan, Italy).

    Article Title: The role of 3D microenvironmental organization in MCF-7 epithelial-mesenchymal transition after 7 culture days.
    Article Snippet: We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations.. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points.. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7.

    Article Title: Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds
    Article Snippet: a Department of Chemistry ‘‘G.. Ciamician’’ and National Consortium of Materials Science and Technology (INSTM, RU Bologna), University of Bologna, Bologna, Italy b Laboratory of Cellular and Molecular Engineering ‘‘S.. Cavalcanti’’, University of Bologna, Cesena, Italy c Health Sciences and Technologies – Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano dell’Emilia, Italy d National Institute for Cardiovascular Research (INRC), University of Bologna, Bologna, Italy e Department of Anaestesiological and Surgical Sciences, University of Bologna, Bologna, Italy f Consiglio Nazionale delle Ricerche – Istituto per lo Studio dei Materiali Nanostrutturati (CNR–ISMN), Bologna, Italy g Laboratorio di NanoBiotecnologie (NaBi) – Istituto Ortopedico Rizzoli, Bologna, Italy h Clinical Department of Radiological and Histocytopathological Sciences, University of Bologna, Bologna, Italy

    Microscopy:

    Article Title: Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells.
    Article Snippet: After washing, samples were stained with AlexaFluor-488 or AlexaFluor-546 secondary antibodies in the dark, counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milano, Italy).

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: Finally, after washes, the samples were mounted and nuclei counterstained with Pro Long anti-fade reagent with DAPI (Molecular Probes, Milan, Italy).

    Article Title: The role of 3D microenvironmental organization in MCF-7 epithelial-mesenchymal transition after 7 culture days.
    Article Snippet: We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations.. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points.. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7.

    Article Title: Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds
    Article Snippet: a Department of Chemistry ‘‘G.. Ciamician’’ and National Consortium of Materials Science and Technology (INSTM, RU Bologna), University of Bologna, Bologna, Italy b Laboratory of Cellular and Molecular Engineering ‘‘S.. Cavalcanti’’, University of Bologna, Cesena, Italy c Health Sciences and Technologies – Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano dell’Emilia, Italy d National Institute for Cardiovascular Research (INRC), University of Bologna, Bologna, Italy e Department of Anaestesiological and Surgical Sciences, University of Bologna, Bologna, Italy f Consiglio Nazionale delle Ricerche – Istituto per lo Studio dei Materiali Nanostrutturati (CNR–ISMN), Bologna, Italy g Laboratorio di NanoBiotecnologie (NaBi) – Istituto Ortopedico Rizzoli, Bologna, Italy h Clinical Department of Radiological and Histocytopathological Sciences, University of Bologna, Bologna, Italy



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    Image Search Results


    Tumour-infiltrating immune cells following CED of cisplatin and/or GL-GM immunizations. Frozen brain sections were stained for CD8 + , F4/80 + and CD206 + cells and the percentage of stained area was determined. The median and range of each group are shown. ( A ) Tumour infiltration of CD8 + cells was increased in GL-GM-immunized mice compared with non-treated (* p = 0.0286) and cisplatin-treated mice (* p = 0.0159). ( B ) Representative staining of CD8 (green) in tumours. ( C ) Tumour infiltration of F4/80 + cells was reduced in GL-GM-immunized mice compared with non-treated mice, both as a monotherapy (non-treated vs. GL-GM, * p = 0.0286) and in combination with cisplatin (non-treated vs. cisplatin + GL-GM, * p = 0.0159). ( D ) Representative staining of F4/80 (red) in tumours. ( E ) The majority of F4/80 + (red) cells expressed CD206 (green). ( B , D , E ) Show representative stainings from one animal of each treatment group. Images were taken at 10x magnification. DAPI was used as a nuclear staining.

    Journal: Scientific Reports

    Article Title: The effect of locally delivered cisplatin is dependent on an intact immune function in an experimental glioma model

    doi: 10.1038/s41598-019-42001-7

    Figure Lengend Snippet: Tumour-infiltrating immune cells following CED of cisplatin and/or GL-GM immunizations. Frozen brain sections were stained for CD8 + , F4/80 + and CD206 + cells and the percentage of stained area was determined. The median and range of each group are shown. ( A ) Tumour infiltration of CD8 + cells was increased in GL-GM-immunized mice compared with non-treated (* p = 0.0286) and cisplatin-treated mice (* p = 0.0159). ( B ) Representative staining of CD8 (green) in tumours. ( C ) Tumour infiltration of F4/80 + cells was reduced in GL-GM-immunized mice compared with non-treated mice, both as a monotherapy (non-treated vs. GL-GM, * p = 0.0286) and in combination with cisplatin (non-treated vs. cisplatin + GL-GM, * p = 0.0159). ( D ) Representative staining of F4/80 (red) in tumours. ( E ) The majority of F4/80 + (red) cells expressed CD206 (green). ( B , D , E ) Show representative stainings from one animal of each treatment group. Images were taken at 10x magnification. DAPI was used as a nuclear staining.

    Article Snippet: Sections were washed, incubated with the secondary antibody for one hour, goat-anti-rat Alexa Fluor 488 IgG (2 mg/ml, Invitrogen) and donkey-anti-rat Alexa Fluor 549 IgG (2 mg/ml, Invitrogen), goat-anti-rabbit Alexa Fluor 488 IgG (2 mg/ml, Invitrogen) and mounted with Pro-long Gold anti-fading reagent containing DAPI (Molecular Probes) for nuclear staining.

    Techniques: Staining